Microbiology: Laboratory Theory & Application, Brief 3rd Edition

Overview:

Microbiology: Laboratory Theory and Application, Brief is an essential laboratory manual designed for introductory microbiology courses, catering to both majors and non-majors. This streamlined version of the best-selling manual is ideal for allied health students or courses that prefer a focused number of experiments. The manual is presented in full color and provides clear, step-by-step guidance on over 60 laboratory procedures. It covers fundamental laboratory skills, microbial growth, microscopy, differential tests, and microbial genetics. This comprehensive yet concise resource is a practical tool for mastering microbiology lab techniques and understanding the theory behind them.

Key Features:

• Targeted Content: Focuses on core experiments and procedures suitable for a brief course structure.
• Full-Color Format: Enhances understanding with vibrant illustrations and photographs.
• Comprehensive Coverage: Includes sections on fundamental skills, microbial growth, microscopy and staining, selective media, differential tests, and microbial genetics.
• Safety Emphasis: Thorough introduction to lab safety, contamination control, and safe handling of microorganisms.
• Practical Application: Emphasizes real-world applications with exercises like antibiotic susceptibility testing and microbial identification.

Table of Contents:

1. Introduction: Safety and Laboratory Guidelines
2. Fundamental Skills for the Microbiology Laboratory
   • Hand Hygiene
   • Aseptic Transfers and Inoculation Methods
3. Microbial Growth
   • Ubiquity and Diversity of Microorganisms
   • Aerotolerance and Effects of Environmental Factors
4. Microscopy and Staining
   • Light Microscopy
   • Differential and Structural Stains
5. Selective Media
   • Media for Isolation of Gram-Positive Cocci and Gram-Negative Rods
6. Differential Tests
   • Biochemical Tests, Fermentation Tests, Nutrient Utilization Tests
7. Quantitative Techniques
   • Environmental Sampling, Standard Plate Count
8. Medical, Environmental, and Food Microbiology
   • Antimicrobial Susceptibility Testing, Water Quality Testing
9. Microbial Genetics and Serology
   • DNA Extraction, Bacterial Transformation, ELISA
10. Identification of Unknowns

• Identification of Enterobacteriaceae, Gram-Positive Cocci, Gram-Positive Rods

Appendices:

• Biochemical Pathways
• Transfer Methods
• Medium, Reagent, and Stain Recipes